If the pitcher HESITATED because of the batter holding up his hands, that could cause his rhythm to be out of place and he throws one down the middle instead of high (example). So yes, if i think the pitcher hesitated in any way because the batter put his hand up (per rule), i would call that hit back and give the batter a strike.
However, i await Bob J or Rich I input, as i tend to following what they teach
