Appeal

[mach3]

I do ISF but an ASA view is also appreciated!

When do you consider a live ball appeal to be made? When the base is tagged? When the fielder starts to speak it out? When the fielder has verbalized the appeal?

I am not talking about an obvious appeal situation, as a lead on a fly ball and the return after the catch) but about a situation, when the runner tries to correct the base running mistake and defence tries to appeal it. Both the runner and the fielder w/the ball are in the vicinity of the base the mistake occurred.

E.g.:
R1, R3. 2 Out. The Batter hits a ball to right field, R1 rounds 2B and misses it. She stops and tries to return to the base as the defence throws the ball there. R3 reaches home savely.
What has the defence to do to appeal the missed base and nullify the run?

THX

Raoul