If the 40-second clock is used, the R does not need to give a RFP signal in most cases. The exception would be if the 40-second clock starts on the R's signal (after a 10-second runoff, injury to Team B, and a few others).
The R will give a 'start clock' signal if necessary (run OOB, first down, etc), which may or may not be accompanied by a whistle (check local listings).
The RFP signal is still used when the 25-second clock is in use.
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